Survey of accessory gene regulator (agr) groups and TSST-1 encoding gene (tst) in Staphylococcus aureus isolated from ewes with mastitis in the northwest of Iran

Authors

  • H. Dastmalchi Saei Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
  • H. Mohammad-Zadeh Graduated from Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
  • S. Aghdasi Graduated from Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract:

Accessory gene regulator (agr locus) is a global regulator of many virulence gene expressions inStaphylococcus aureus. Four interference classes related to genetic polymorphisms in the agr locus have sofar been described. In the present study, the agr locus specificity groups were studied within a total of 43 S.aureus isolates which were isolated from ewes with mastitis in three regions in the Northwest of Iran. Inaddition, the isolates were examined for the presence of TSST-1 (toxic shock syndrome toxin-1) encodinggene (tst) using PCR. Identification of agr groups, using agr group-specific multiplex PCR, classified themajority of isolates into group I (51.16%) and to a lesser extent into agr group IV (44.19%). Only one isolate (2.23%) for each of the agr groups II and III was found. This study also indicated that 44.19% of isolates (n=19) possessed the tst gene and that 94.74% (n=18) belonged to agr group I. According to the results, certain agr groups comprised the great majority of sheep mastitis associated S. aureus isolates. Moreover, the high proportion of S. aureus isolates was tst positive, indicating an issue requiring consideration as it is relevant to food hygiene. The results also showed a variation in distribution of the tst gene among the different agr groups.

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Journal title

volume 14  issue 4

pages  334- 340

publication date 2013-12-30

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